ABSTRACT
Objective: To explore the potential anti-coronavirus mechanism of Xiaoer Feire Kechuan Particles (XFKP). Methods: TCMIP database was used to search and summarize the information of each medicinal herbs-components-target of XFKP. All the candidate target genes were also searched. "Corona virus" was used as the key word in Genecards database to search for corona virus related targets, and the mapping of the active targets with XFKP were used to select the common targets as the research targets. The selected common targets will be found in the summarized database of each medicinal herbs-components-target. The common targets were imported into the STRING database to construct the network diagram of target interaction, and Cytoscape 3.3.0 software was used for visualization processing to screen out the core targets. With the help of OmicsBean analysis platform and String database, Gene ontology (GO) biological process analysis and KEGG signal pathway enrichment analysis were carried out on the target. Results: A total of 342 chemical components and 737 candidate target genes were obtained. Venny mapped 48 common targets, corresponding to 173 compounds, 19 core targets, and 27 main compounds. GO biological process (BP) entries included 3 420, cell component (CC) entries included 239, and molecular function (MF) entries included 343. Through KEGG enrichment analysis, 160 signal pathways related to the therapeutic effect of XFKP were obtained. The "medicinal herbs-components-target-pathway" network of the key targets of XFKP was established. Conclusion: The 173 compounds can intervene 160 signaling pathways by acting on 48 related targets, such as IL-17 signaling pathway, influenza A, etc., mainly involving anti-inflammation, immune regulation, relieving cough and asthma, antibacterial, antiviral and sedative aspects, providing theoretical basis for the treatment of corona virus-related diseases with XFKP.
ABSTRACT
Objective: To explore the potential mechanism of Xiahuang Granules in the treatment of opioid-induced constipation. Methods: Various medicinal ingredients and targets information of Xiahuang Granules were found in TCMSP database. In Genecards database, "opiod constipation", "opioid-induced bowel dysfunction" and "opioid-induced constipation" were used as keywords to search for targets related to opioid-induced constipation, and the active targets mapping of Xiahuang Granules were selected as the research targets. The common targets were imported into the STRING database to build the targets interaction network diagram, and Cytoscape 3.3.0 software was used for visual processing to screen out the core targets. The OmicsBean analysis platform and STRING database were used to conduct GO function enrichment and KEGG pathway enrichment analysis on the targets. Results: A total of 55 chemical constituents, 158 candidate target genes, 86 common targets after mapping Venny, 49 corresponding chemical components, 12 core targets and 19 main chemical components of Xiahuang Granules were obtained by screening. GO functional enrichment analysis showed 4 150 biological process items, involving chemical stimulus cell reactions, chemical reactions, biological quality control and other processes; A total of 302 cell composition items, involving voxel projection, extracellular space, and whole membrane processes; A total of 459 molecules function items, involving processes such as protein binding, molecular transduction activity, and enzyme binding were obtained. KEGG enrichment analysis revealed 149 signaling pathways related to the effect of Xiahuang Granules, involving the AGE-RAGE signaling pathway in diabetic complications and tumor necrosis factor signaling pathway, etc. The network of "medicinal herb-component-target-pathway" of Xiahuang Granules was established. Conclusion: The main chemical components of Xiahuang Granules including naringenin, nobiletin, aloe emodin, rhein may regulate endocrine resistance and tumor necrosis factor signaling pathways by acting on key proteins such as TNF, MAPK3, IL-6, VEGFA, and PTGS2, thus play a role in laxative, antispasmodic, and promoting gastrointestinal motility, which provides theoretical basis for Xiahuang Granules to treat opioid-induced constipation and is consistent with the preliminary verification results of Xiahuang granules.